The collection of subgingival plaque samples is the common way for the determination of periodontopathic bacteria. However, recently, salivary analysis has been discussed as an advantageous future diagnostic method for periodontitis because it offers simple quantitative sampling and the possibility to assess various bacteria.

2020-04-01

All participants were requested to refrain from food for 8 h and oral hygiene (brushing or flossing the teeth) for 12 h before sampling. 2020-04-01 Clinical examination of periodontal parameters and collection of saliva and pooled subgingival plaque samples from mesial-buccal sites of 4 first molars were performed before initial therapy and 2, 4 and 6 months respectively after mechanical therapy, and saliva … Subgingival plaque was then collected with sterile paper points (Meta Biomed ® Co. Ltd, Cheongju, South Korea) inserted into the gingival sulcus of Ramfjord’s index teeth for 15 seconds(7-9). All six paper points from each patient were pooled in a micro centrifuge tube and stored at -200C until laboratory testing. Lab procedure DNA extraction: Plaque samples were let to thaw at 2015-04-01 Sample Collection. Buccal and subgingival plaque samples were collected 1 week after the full-mouth periodontal examination. All participants were requested to refrain from food for 8 h and oral hygiene (brushing or flossing the teeth) for 12 h before sampling.

Subgingival plaque collection

Bretz et al. (1989) demonstrated that the supragingival plaque does not interfere with the results of BANA hydrolysis. subgingival plaque can be successfully cultured, but the deeper layer may be missed [10]. In comparison, curettes can collect subgingival plaque from the entire periodontal pocket, but the active periodontal pathogens in the outer layer of the plaque can be underestimated with this method [11]. 2020-04-24 2000-08-01 subgingival plaque. Technically, local environmental changes related to orthodontic band and brackets may influence the bacterial species in periodontal plaque. However, it seems necessary to assess variations in subgingival plaque caused by orthodontic appliances.

Sample collection, DNA extraction, library prep, and sequencing. Plaque sample collection and DNA extraction were conducted as specified in reference 27. Libraries were prepared using the NEBNext Illumina DNA library preparation kit according to manufacturer’s specifications (New England Biolabs, Ipswich, MA).

1. MID (Motivation, Instruktion och MID, plaque registrering, fickstatus, mobilitet, furkar, BVS. 2.

plaque. plaque [plak], medicinsk och odontologisk term, se plack. (8 av 8 ord). Vill du få tillgång till hela artikeln? Testa NE.se gratis eller Logga in

The samples were immediately placed at 4 C and analyzed within 4 h. Define subgingival plaque. subgingival plaque synonyms, subgingival plaque pronunciation, subgingival plaque translation, English dictionary definition of subgingival plaque. n.

After the removal of supragingival plaque. subgingival plaque demonstrated columnar ar-rangement of morphologically distinct bacterial species from the tooth surface to the outer surface of the plaque (Listgarten et al. 1975). Subgingival plaque was frequently characterized by a zone of gram negative and/or motile species located adjacent to the epithelial lining of the pocket while gram positive Subgingival plaque sample collection.
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Dental Calculus/analysis* Dental Plaque/analysis* Humans; Methods; Models, Structural; Specimen Handling A Method for Collection of Subgingival Plaque and Calculus.

J Bacteriol 2001;183:3770-83.
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Subgingival plaque samples are also collected for microbiological analysis of the microbiota with focus on hydrogen sulfide producing bacteria. Samples are

Supragingival plaque was first removed from the sample teeth with sterilized Gracey curettes and gauze. The site was then cleaned and isolated using cotton roles and air dried gently. The collection of subgingival plaque samples is the common way for the determination of periodontopathic bacteria. However, recently, salivary analysis has been discussed as an advantageous future diagnostic method for periodontitis because it offers simple quantitative sampling and the possibility to assess various bacteria. Plaque collection Supragingival plaque deposits were removed from the gingival margin, which was dried with blast of air prior to sampling.